
Using ONT direct RNA sequencing to assess WAS vectors. Plots showing the sequencing coverage (green), location of the 3′ ends of reads (orange), and splicing patterns (purple lines connecting splice donors and acceptors) for four different WAS vectors sequenced by ONT direct RNA sequencing: (A) WAS LV1, (B) WAS LV2, (C) WAS LV3, (D) WAS LV4. (E) Reference sequence of the WAS vector, with various features annotated.











