Figure 1.
Comparison of three different long-read RNA sequencing approaches for lentiviral vector QC. Sequencing coverage (green), location of the 3′ ends of reads (orange), and splicing patterns (purple lines connecting splice donors and acceptors, with numbers indicating how many reads support each splicing event) for three different long-read sequencing technologies. (A) PacBio cDNA sequencing data. (B) ONT direct RNA sequencing data. (C) ONT direct cDNA sequencing data. (D) Reference sequence of the Globin LV, with various features annotated.
