Figure 4.
Improving the flexibility of TD-PE by fine-tuning pegRNA design. (A) Schematic diagram showing the design of TD-PE with additional insertions. Insertions with different lengths (2–10 nt; shown in bold pink and green) were added between the original RT-template and the PBS so as to prolong the original duplications and relax the requirement of the nick position. (B) Agarose gel electrophoresis analysis of the presence of TD at the HEK3 and AAVS1 loci. (C) Quantification of the efficiency of targeted TD in B. Values and error bars reflect mean ± SD of n = 3 independent biological replicates.
