Overview of editing strategies for TD-PE. Schematic diagram showing the design and the putative editing process of TD-PE strategy. The TD-PE strategy contains a pair of in trans pegRNAs, pegRNA1 and pegRNA2, which are shown in red and orange, respectively. Each pegRNA contains an RT-template that is homologous to the sequence of the 5′ end or 3′ end of the fragment to be duplicated. These homologous regions are marked by colors that are the same as their corresponding pegRNAs. A blue line indicates the fragment between the homologous region. The prime edited ssDNA strands reannealed to form an intermediate that contains partial tandem duplication (TD), which was then repaired by endogenous DNA repair mechanisms to accomplish the TD.
