Pol IV–suppressed sRNAs are conserved in Arabidopsis. (A) sRNA northern blots showing the levels of Pol IV–suppressed sRNAs (SP. Locus1) and other sRNAs from Arabidopsis seedlings and inflorescence tissues. (B) Boxplots showing the sRNA abundance from different genotypes of Arabidopsis with counts from Pol IV–dependent and –suppressed 23- to 24-nt size class bins. The sRNA data sets were obtained from GEO GSE165574, GSE45368, and GSE99694. Analyses depicts sRNAs from inflorescence tissues. (C) Boxplots showing the sRNA abundance from different genotypes of Arabidopsis with counts from Pol IV–suppressed 23- to 24-nt size class bins depicted in B. The sRNA data sets were obtained from GEO GSE79780 (inflorescence tissues). The y-axis is scaled to the inverse sine hyperbolic function of RPKM values. Mann–Whitney U test was used to test statistical significance against WT R1, unless stated otherwise. (*) P-value < 0.0001. (D) Bar plots showing the abundance of Pol IV–dependent and –suppressed sRNAs of 16- to 35-nt size range.
