Figure 1.
Experimental design features of a STARR-seq experiment. (A) Variations of pre-experimental design features and their impact on library preparation protocol are shown. (B) Fragment length (mean and range are shown) of published STARR-seq libraries reported by deidentified papers A to X (on x-axis) and reported fragment length (on y-axis) are shown. Note that studies W, M, O, P, Q, R, and G reported the range for their fragment lengths indicated by the error bars, and other studies reported the exact length of the fragment. (C) Read architecture schematic of a STARR-seq plasmid library (top) and “input” and “output” sequencing libraries (bottom) are shown.
