The impact of SWI/SNF and NuRD inactivation on gene expression is tightly coupled with levels of RNA polymerase II occupancy at promoters

SWI/SNF modulate the rates of Pol II release into the gene body to paradoxically affect the expression of genes having average/high Pol II promoter occupancy levels. (A) Changes in Pol II initiation (left) and Pol II release (right) levels at gene promoters (N = 6387) in mESCs upon inhibiting BRG1 activity by BRM014. Also shown are the changes in the nascent RNA levels of these genes upon BRG1 inhibition (far right). (B) Changes in Pol II initiation (top) and Pol II release (bottom) at genes stratified based on changes in their expression upon inhibition of BRG1 activity by BRM014 in mESCs. (C,D) Genome browser view for Cdk1, where inhibition of BRG1 activity using BRM014 leads to a decrease in Pol II initiation at the promoter (C) and an increase in Pol II release into the gene body (D).