De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

Antisense transcription in the testis. (A) Genome browser screenshot of Actb locus showing strong antisense transcription in all cell types profiled. Green indicates H3K4me3 ChIP-seq; blue, (+) strand RNA; and red, (−) strand RNA. (B) Scatter plot showing exonic log₂(RPKM) versus antisense log₂(RPKM) in testicular cell types (this study) and the liver (from Wang et al. 2020). (C) Boxplot showing log₂(RPKM) of exonic (red) and antisense (blue) sequences binned by levels of H3K4me3 in testicular cells and the liver. (D) Heatmap showing levels of exonic and antisense RNA and ChIP-seq enrichment for H3K4me3 at TSS ±3 kb in testicular cells types and the liver.