3D chromatin structures enhance transcriptional activity in the eight-cell stage. (A) The bar plot shows the percentage of TAD boundaries (GSE82185) that are new in the current compared with the previous stages. (B) The average expression level of genes is located in the TAD boundary and outside the TAD boundary (GSE82185). P-values were calculated by t-test. (C) Pie charts show genes with accessible chromatin (GSE66581) in the promoter or the chromatin interaction loop region (GSE82185). (D) The expression level of Sidt2. (E) Hi-C heatmaps of Sidt2 and associated loop region at 5-kb resolution for late two- and eight-cell embryos. (F) UCSC plots show chromatin accessibility (GSE66581) of Sidt2 and the associated significant interaction region. (G) The model shows that accessible chromatin in the eight-cell stage is condensed by widespread interaction loops, thus enhancing transcriptional activity.
