Distal H3K27me3 resetting during porcine preimplantation development. (A) Heat maps and aggregate profile plots showing the dynamics of distal H3K27me3 in porcine oocyte PMDs. PMDs were ordered descending by their H3K27me3 intensities in MII oocytes. (B) A genome browser snapshot showing the removal of distal H3K27me3 in porcine oocyte PMDs upon fertilization, and the H3K27me3 re-establishment in porcine morulae and blastocysts. DNA methylation levels (mCG) in oocyte and spermatozoa are also shown. (C) Heat maps and aggregate profile plots showing the dynamics of distal H3K27me3 established in porcine two- to four-cell embryos. (D) A genome browser snapshot showing the establishment of distal H3K27me3 in porcine two- to four-cell embryos, which was nearly not observed in either oocytes or embryos of other stages. DNA methylation levels (mCG) in oocyte and spermatozoa are also shown. (E) Pie chart showing the genomic distribution of distal H3K27me3 established at the two- to four-cell stages. (F) Heat maps showing the transcriptional levels (FPKM) of porcine ZGA genes and genes covered by H3K27me3 on their gene body regions in porcine oocytes and early embryos.
