Profiling of H3K4me3 and H3K27me3 in porcine oocytes and early embryos. (A) Experimental design of the developmental stages at which samples were collected for H3K4me3 and H3K27me3 ULI-NChIP-seq analyses. Samples are porcine germinal vesicle (GV) and metaphase II (MII) oocytes, and in vitro fertilized (IVF) embryos at pre-ZGA (zygotes [1C] and two-cell embryos [2C]), peri-ZGA (four-cell [4C] and eight-cell [8C] embryos), and post-ZGA stages (morulae [Mo] and blastocysts [Bl]). (B) Line plots showing the percentage of genomic regions accumulated by H3K4me3 and H3K27me3 in distal and promoter regions. (C) Genome browser snapshots showing the dynamics of H3K4me3 and H3K27me3 in porcine oocytes and early embryos, and DNA methylation levels (5-methylcytosines in CpG [mCG]) in porcine oocyte and spermatozoa. Genomic regions containing oocyte partially methylated domains (PMDs, left panel) and gene promoters (right panel) are shown.
