Parent-of-origin-dependent ASE patterns fall into three expression profiles. (A) Proportion of ASE genes per tissue with each parental bias profile. Heatmaps of ASE profiles across analyses: (B) tissue-independent, (C) tissue-dependent, and (D) context-dependent. A subset of the 271 genes is shown, including those validated with pyrosequencing. Genes are color coded by their expression pattern in each tissue-by-context analysis. Shades of red and blue indicate their degree of maternal or paternal bias, respectively (POE scores). If genes are not biased, shades of yellow indicate their biallelic expression levels (log-transformed total counts). Black indicates genes are not expressed. Bolded genes are canonically imprinted. The y-axis is grouped and sorted by chromosomal position. Supercolumns denote tissues: (HYP) hypothalamus; (WAT) white adipose; and (LIV) liver. Subcolumns denote environmental contexts: (All) all contexts; (H) high fat; (L) low fat; (F) females; (M) males; (HF) high fat females; (HM) high fat males; (LF) low fat females; and (LM) low fat males. (E) POE scores for each parental bias profile. Vertical lines indicate mean POE scores. Dots represent individual ASE genes. (F) UpSet plots of the significant sex, diet, and/or sex-by-diet effects of context-dependent genes in each tissue. Bar height and color indicate how many genes with each parental bias: (red) maternal; (blue) paternal; and (yellow) direction switching.
