Identification of the sex chromosomes by RAD-tag sequencing. (A) Distribution of RADSex markers between males (horizontal axis) and females (vertical axis) using a minimum depth of 1 to consider a marker present in an individual. There were 892 markers significantly associated with male phenotypes in the tiles highlighted in red. (B) Manhattan plot showing the negative log of P-value of association with sex for all RADSex markers aligned to the genome. The vast majority of aligned markers significantly associated with sex (97%) was aligned to scaffold_20. (C) Negative log of P-value of association with sex for all RADSex markers aligned to scaffold_20. Markers significantly associated with sex aligned to a continuous region spanning from the start of the chromosome to ∼32.5 Mb.
