The abundances of 21U RNAs and 22G RNAs targeting coding genes are significantly higher in the bB2 embryos than in the nB2 embryos. (A) Scatter plot showing pairwise comparison of normalized read counts of the Caenorhabditis briggsae- (blue) or Caenorhabditis nigoni-specific 21U RNAs (pink) between bB2 and nB2 embryos. Note that the overall abundances of 21U RNAs are apparently higher in the bB2 than in the nB2 regardless of their parent-of-origin. (B) Box plots showing the normalized read counts of C. briggsae- (blue) or C. nigoni-specific 21U RNAs (pink) in the bB2 and nB2 embryos. P values of pairwise comparison with Wilcoxon ranked-sum test is indicated. (C) Scatter plot showing the pairwise comparison of normalized read count of 22G RNAs targeting orthologous coding genes between the bB2 and nB2 embryos. The numbers of genes with a significantly higher abundance of targeting 22G RNAs in the bB2 relative to the nB2 embryos (purple) or vice versa (green) are indicated. (D) Venn diagram showing the number of coding genes targeted by the 22G RNAs that were significantly up- (top) and down-regulated (bottom) in the bB2 or nB2 embryos relative to both parents.
