Arrays of Sir3 are sufficient to promote trans interactions. (A) Schema describing the strains used in the assay. sir3Δ rap1-17 strains carrying a lac operator (lacO) array at the LYS2 locus on Chromosome II and a lacO array at the LEU2 locus on Chromosome III are expressing either GFP-LacI or GFP-LacI-Sir3. Merge image of a transmitted-light image and the GFP channel fluorescent image of a strain expressing the GFP-LacI-Sir3 construct (yAT1470). (B) Graphic showing the percentage of cells with one spot in different strains and conditions: GFP-LacI (yAT1476), GFP-LacI-Sir3 (yAT1470) in the presence or in the absence of splitomycin (a Sir2 inhibitor) and GFP-LacI-Sir3 sir4Δ (yAT1864). Cells were grown in CSM 2% glucose to mid-exponential phase of growth before being imaged. (C) Schema representing the nuclear organization of strains expressing either the GFP-LacI (left) or the GFP-LacI-Sir3 (right).
