Sir3 spreading coincides with telomere–telomere contacts. (A) Contact probability as a function of genomic distance Pc(s) (log scale) for different strains either in G1 or G2. (B) Accumulated normalized contacts over 40-kb subtelomeric windows (0 = X-core elements). (C) Sir3 enrichment over 40-kb subtelomeric windows (data from Hocher et al. 2018). (D) Pile-up contact maps ratio for various pairs of strains of subtelomeric windows centered on the extended silent domains (ESD; dotted line) determined in the SIR3 overexpression (OE) condition. The windows extend over 50 kb in both directions from the ESD. (E) Representative inter-chromosomal ratio maps of the same pairs of strains as in D processed through Serpentine binning. Sir3 enrichment observed by ChIP (Hocher et al. 2018) in the indicated conditions is plotted along the top and right axis. (F) Schemas illustrating the behavior of telomeres and subtelomeric regions in the SIR3 OE, WT, and sir3-A2Q OE conditions.
