Expression of PRAM transcripts correlate with the neighboring gene Pik3cg in mouse and human. (A) Expression levels of PRAM transcripts and their neighboring genes in untreated (Unt) and 48-h ß-estradiol (ß-est)-treated G1E-ER-GATA1 cells. CUFFp.chr12.15498's isoform 1 was not detected by semi-qRT-PCR and thus was not measured here. Two-tailed Student's t-test; (**) P-value < 0.01, (***) P-value < <0.001. (B) Fold changes of PRAM mouse transcripts and their neighboring genes in the RNA-seq data sets of untreated and 48-h ß-estradiol-reated G1E-ER-GATA1 cells (G1E) and wild type versus deletion of Gata2 +9.5 enhancer aorta-gonad-mesonephros (AGM). (C) Counterparts of PRAM mouse transcripts in human with their supporting genomic features. (D) Semi-qRT-PCR measurement of PRAM human transcripts and their neighboring genes. Gene model name prefixes were removed for brevity. (E,F) Correlation of gene expression levels between CUFFm.chr7.6148 with PIK3CG and PRKAR2B in K562 cells (E) and TCGA-LAML patients (F).
