Morphology of wild-type and sORF-encoded peptide mutant lines. The phenotypes of psep25 KO and PSEP25 OE lines grown on BCDAT medium: (A,D) wild type; (B,E) knockout of PSEP25; (C,F) overexpression of PSEP25. (G) Diameter of moss plants with knockout of PSEP25 (Supplemental Fig. S13J–M; Supplemental Table S6). (H) Diameter of moss plants with overexpression of PSEP25 (Supplemental Fig. S14M–P; Supplemental Table S6). (I,J,M) Number of leafy shoots in wild-type and three psep25 KO lines. (K,L,N) Number of leafy shoots in wild-type and two PSEP25 OE lines on BCD medium. Arrows show young leafy gametophores. The phenotypes of psep18 KO and PSEP18 OE lines grown on BCD medium with 0.5% glucose: (O,R,T) wild type; (P) knockout of PSEP18. (Q) Diameter of moss plants with knockout of PSEP18 (Supplemental Fig. S16A–C; Supplemental Table S6). (S,U) Overexpression of PSEP18. (V) Diameter of moss plants with overexpression of PSEP18 (Supplemental Fig. S16D–F; Supplemental Table S6). Scale bar: 0.5 mm. P-value was calculated by Student's unpaired t-test. (****) P-value < 0.0001, (***) P-value < 0.001, (**) P-value < 0.01, (*) P-value < 0.05.
