Figure 4.
Efficiency of SNGD-mediated gene editing using various types of repair templates. (A,D) Diagrams of the repair templates. (B,C,E) Nucleotide substitution efficiency as measured by flow cytometry (mean ± SD, N = 3) is presented in each panel. The EGFPcC>G reporter was nicked at the sgEGFP332s site, and donor plasmids were nicked at the sgUC57N2 site. The indicated repair template was used as a plasmid donor in each assay. 293T EGFPcC>G reporter cells were used in B and E. HeLa EGFPcC>G reporter cells were used in C.
