Figure 1.
Nucleotide substitution by tandem nick (TN) of the reporter gene. (A) Schematic of the nucleotide substitution reporter assay. Representative data from flow cytometry and epifluorescent images of 293T EGFPcC>G reporter cells are also presented. (B) Diagram of the EGFPcC>G reporter and sgRNAs. (C) Diagram of the repair templates. (D) Nucleotide substitution efficiency as measured by flow cytometry (mean ± SD, N = 3) is presented. The indicated sgRNAs were expressed with Cas9D10A. The indicated repair templates were introduced into the cells as plasmid donors. Also see Supplemental Figure S1.
