HD-Marker: a highly multiplexed and flexible approach for targeted genotyping of more than 10,000 genes in a single-tube assay

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Figure 4.
Figure 4.

Rarefaction analysis of HD-Marker libraries at different sequencing scales. For 296-plex (A), 795-plex (B), 1293-plex (C), and 12,472-plex (D), loci detection is saturated at 0.08, 0.2, 0.6, and 2 million reads, respectively, and 98.9%, 98.6%, 98.5%, and 97.5% of genotyping accuracy can be achieved at the optimal sequencing depths.

This Article

  1. Genome Res. 28: 1919-1930

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