Transcriptome evidence reveals enhanced autophagy-lysosomal function in centenarians

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Figure 4.
Figure 4.

Functional studies of up-regulated autophagy-lysosomal genes in IMR-90 cells and transgenic flies. (A) Protein levels of SQSTM1 in IMR-90 cells with overexpressed CTSB, ATP6V0C, ATG4D, and WIPI1, respectively. (B) The SA-β-Gal staining cell ratio in IMR-90 cells with overexpressed CTSB, ATP6V0C, ATG4D, and WIPI1, respectively. (C) Cumulative cell population doubling of IMR-90 with overexpressed CTSB, ATP6V0C, ATG4D, and WIPI1. (D) Protein levels of CDKN2A and CDKN1A in IMR-90 cells with overexpressed CTSB, ATP6V0C, ATG4D, and WIPI1, respectively. (E) Diagram of Gal4-UAS system in flies. (F) Survival curve of Drosophila with overexpressed Atg18a. (*) P < 5.0 × 10−2. (OE) Overexpression.

This Article

  1. Published in Advance October 23, 2018, doi: 10.1101/gr.220780.117 Genome Res. 28: 1601-1610

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