Comparison of solid-phase reverse and liquid-phase transfection for CRISPR/Cas9-mediated gene editing. The bar chart shows gene editing efficiencies (produced indels) after solid-phase reverse and liquid-phase cotransfection of recombinant Cas9 with gRNAs in HeLa (A) and HEK293T cells (B). gRNAs targeting PLK1 (light gray), KIF11 (dark gray), and INCENP (black) were analyzed. For comparison reasons, we included all-in-one cDNAs encoding the respective gRNAs and delivered by solid-phase reverse transfection. For clarity, we summarized the performance of the negative controls in Supplemental Table S3. All transfection reactions were performed in 384-well plates. Each target was knocked out in triplicates. The cell lysates of the triplicates were collected in one single tube for the indel analysis.
