Comparison of protein localization after solid-phase reverse antibody transfection and immunofluorescent staining in HeLa cells. (A) The intracellular distribution of transfected GFP. The upper images show the localization of transfected anti-GM130 (B), anti-alpha tubulin (C), and anti-KIF11 (D) antibodies. The lower images show the results of the IF staining using the same antibodies. (E) Transfection efficiency of antibodies and GFP. Bars represent average transfection efficiency derived from six independent replicas with every antibody shown and 14 independent replicas with GFP; error bars represent the standard deviations. GFP, anti-KIF11, and anti-alpha tubulin antibodies were transfected with Lipofectamine RNAiMAX, using reaction condition I for GFP and condition VIII for antibodies. Anti-GM130 antibody was transfected with Lipofectamine 3000 using condition VI (Supplemental Table S1). The transfections were performed in 384-well plates. Nuclei were counterstained with Hoechst 33342 dye. (Scale bar) 20 µm.
