Distribution of H3K27me2/3 is mostly unaffected by elimination of DNA methylation, but loss of H3K9me3 in the dim-5 mutant causes redistribution of H3K27me2/3 to constitutive heterochromatin. ChIP-seq tracks of H3K27me2/3 in wild-type, dim-5, and dim-2 strains are displayed in dark blue, and tracks of H3K9me3 are displayed in green (inverted; shown for the wild-type strain only). While genome-wide redistribution of H3K27me2/3 was observed in a dim-5 strain, the vast majority of H3K27me2/3 domains were unchanged in a dim-2 mutant, with the exception of three novel H3K27me2/3 domains on LG VI, one of which normally sports H3K9me3. The black, light blue, and red lines indicate tracks of H3K27me2/3 ChIP-seq performed in wild-type, dim-5, and dim-2 strains, respectively. Predicted genes are displayed as gray rectangles below the ChIP-seq tracks. The scale bar (500 kb) applies to all seven chromosomes.
