Figure 2.
Comparison of nascent strand densities on Chr 11 from lambda exonuclease and NSCR purifications. A comparison of the nascent strand densities obtained by lambda exonuclease-based purification from wt MEFs in a previous study (Cayrou et al. 2011) and by NSCR from wt or MCM2-deficient MEFs in the present study. (A) An ∼45-kbp region of Chr 11 centered on the Hoxb4 gene; (B) all of Chr 11. For each panel: (1) scale and gene tracks; (2) lambda exonuclease SNS data; (3,5) NSCR-SNS data from wt cells, experiments 1 and 2; (4,6) NSCR-SNS data from MCM2-deficient cells, experiments 1 and 2; (7) total genomic DNA from thymus. In B, percentage GC is included in (1).
