Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

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Figure 7.
Figure 7.

Reduced off-target effects of Cas9-m9R and sgRNA:9R. HEK293T cells were treated with Cas9-m9R and sgRNA:9R once per day for 3 d. Three days after the first treatment, the mutation frequencies at on- and off-target sites were determined using the T7E1 assay. The arrows indicate the expected position of DNA bands cleaved by T7E1. The protospacer adjacent motif is shown in bold. Mismatched bases are underlined.

This Article

  1. Published in Advance April 2, 2014, doi: 10.1101/gr.171264.113 Genome Res. 24: 1020-1027

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