Figure 3.
Cas9-m9R and sgRNA:9R-mediated mutagenesis of an episomal target sequence. (A) Schematic representation of the cell-based reporter assay. (B) Representative flow cytometry of reporter-transfected cells sequentially treated with Cas9-m9R and sgRNA:9R three times or those transfected with plasmids encoding Cas9 and sgRNA as the positive control. The cells were analyzed 3 d after the first treatment with Cas9-m9R and sgRNA:9R or transfection with plasmids encoding Cas9 and sgRNA. The percentages of GFP+ cells are indicated.
