Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

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Figure 1.
Figure 1.

Schematic representation of (A) Cas9 protein conjugated to a cell-penetrating peptide (CPP) and (B) sgRNA complexed with a CPP. The single-letter codes for amino acids are used ([C] cysteine; [G] glycine; [R] arginine; [L] leucine). (His) Histidine tag; (HA) hemagglutinin tag; (Mal) maleimide; (sgRNA) single-guide RNA.

This Article

  1. Published in Advance April 2, 2014, doi: 10.1101/gr.171264.113 Genome Res. 24: 1020-1027

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