mRNA splicing is genetic. (A) The dependence of Apobec3, Clec7a, and Il3ra splicing on the macrophage genetic background is genetically transmissible and is revealed when the PSI values are averaged based on the genotype at the relevant splicing QTL (sQTL). (B) Conversely, the differential splicing of Samhd1 is stimulus-induced and is independent of the macrophage genetic background. Averaging the Samhd1 PSI values in the RI mice based on the genotype at the Samhd1 suggestive sQTL confirms that the splicing of Samhd1 is only variable between the stimuli and not the mouse strains. The AJ allele (AA) is presented as gray bars and the B6 allele (BB) as black bars (mean ± SD, [***] P < 0.001, Student's t-test)
