Annotation of the proline-rich nuclear receptor coactivator 2 locus with ribosome profiling data. (A) This schematic shows the GENCODE annotation for the human PNRC2 locus compared against its mouse and zebrafish orthologs. Annotation for the latter two models is taken from the Vega manual annotation resources (Wilming et al. 2008). Alternative splicing within the 5′ UTR has been omitted for clarity, and intron sizes are not to scale. Equivalent ORFs are shown in yellow and green in each model, with UTR sequences shown in red. The splice donor site of the 5′ UTR exon is conserved between human and mouse; whether this is also true for the 5′ UTR of the zebrafish model cannot be ascertained. The downstream CDS encodes the known PNRC2 protein. The TIS of the upstream ORF is supported by ribosome profiling data in human and mouse, from Lee et al. (2012) and Ingolia et al. (2011), respectively. Data from the latter set also support the TIS of the PNRC2 CDS in mouse. Equivalent RP resources for zebrafish are not available. (B) Alignment of the human, mouse, and zebrafish upstream ORFs, with conserved residues highlighted in red.
