JIL-1, 14-3-3, and CBP are required for enhancer-promoter interactions. 3C analysis of Kc167 cells after Gal knockdown and treatment with ethanol (Non) or 20-HE (20HE) for 3 h was performed in normal cells as well as cells in which the expression of the JIL-1, CBP, 14-3-3, or Gal genes was inhibited by treatment with the corresponding dsRNAs. 3C analysis was also done on cells treated with DRB for 5 min. Cross-linking efficiencies are reported after normalizing to a BAC clone with an insert of 170 kb containing the entire Eip75B locus. A restriction digest of this DNA gives 112 fragments numbered 1 to 112, beginning with the first fragment of the BAC. Error bars represent the standard deviation of the mean of 3 biological replicates.
