Phosphorylation of H3S10 and H3S28 by JIL-1 at promoters and enhancers is necessary for transcriptional activation of ecdysone responsive genes. (A) Structure of three early 20-HE responsive genes with previously characterized enhancers showing the location of promoter, enhancer, and coding sequences used in ChIP analyses. ChIP-chip (modENCODE) profile of RNAPII large subunit indicated in green above each gene. (B) ChIP analysis of Kc167 cells treated for 3 h with ethanol (Non) or 20-HE (20HE). Antibodies used for pull-down are indicated along the x-axis. (Ser5ph) RNAPII phosphorylated on serine 5 of CTD, (Ser2ph) RNAPII phosphorylated on serine 2 of CTD. DNA was quantified by real-time PCR using primers designed to amplify the promoter, enhancer, and coding regions of each of the three genes.
