Figure 5.
Functional effect of candidate driver cancer genes by siRNA-mediated gene silencing in KLE endometrial cancer cell line. KLE cells were transfected with siRNAs targeting the indicated genes. Mock, risc-free siRNA, and nonspecific siRNA served as controls. (A) Efficacy of PTEN siRNA on AKT phosphorylation was determined by Western blotting. Cells transfected with indicated siRNAs were assayed for cell viability (B) 7 d or (C) 5 d post-transfection. Cell viability relative to mock-transfected cells was shown. (*) P < 0.05, compared with mock control.
