Candidate driver cancer genes confirmed by overexpression of wild-type genes or mutants in Ba/F3 viability assay. Ba/F3 cells were transfected with wild-type (WT) or corresponding mutant(s) (mutation sites indicated) of (A) six positive genes in the shRNA screen and (B) 11 genes inactive in the screen. Cells transfected with shRNA were included in the assay as reference. pGIPZ vector is the empty vector carrying shRNA while LacZ corresponds to β-galactosidase in the pLenti6.3 vector. Cells were cultured without IL-3 for 4 wk and harvested for viability assay. Cell viability relative to Ba/F3 parental cells was shown. (*) P < 0.05, compared with Ba/F3 parental control. (#) A significant difference in cell viability between WT and mutant-transfected cells (P < 0.05).
