Pleiotropic effects on morphology. (A,B) Representative micrographs from a hemizygous deletion pair, LSB3 Δy/S (A), and LSB3 Y/Δs (B). Cells are stained with FITC- concanavalin A (green), Alexa Fluor 594 phalloidin (red), and DAPI (blue). (C) Barplot of a single morphological trait, cell size of budded yeast with two nuclei (C101_C), for all RH strain pairs. Error bars, ±1 SE. The work by Ohya et al. (2005) contains a full list of phenotypes and their descriptions. (D) Heatmap of hierarchically clustered nonredundant morphological phenotypes for all RH pairs. Phenotypes are the top principal components of 220 quantitative morphological traits of unbudded (A), budded with one nucleus (A1B), and budded with two nuclei (C) cells. The three leftmost phenotypes primarily correspond to cell size of yeast from each of the three cell types (A, A1B, and C).
