Figure 3.
PRC1 preferentially binds stalled promoters in S2 cells. (A) PRC1-bound promoter exhibits a higher abundance of promoter-proximal short RNA 3′-ends indicative of increased Pol II stalling (Nechaev et al. 2010). (B) PRC1 largely binds promoter producing small RNAs (≥1 read from 5′- and 3′-end libraries). (C) Pol II preferentially remains stalled at PRC1-bound promoter as calculated by the ratio of promoter-proximal occupancy versus gene body. The two populations are significantly different (P-value < 2.2 × 10−16, two-sample Kolmogorov-Smirnov test).
