Novel sequence usage in hESCs. (A) Distribution of novel transcribed clusters compared with Ensembl gene models. (B) IGV view of the PHF10 locus showing expression (scale: 0–250 tags) of an extended internal exon (red shading). (C) IGV view of the OGT locus showing expression (scale: 0–500 tags) of a novel retained intron (blue shading) supported by junction tag evidence. (D) A consensus poly(A) sequence (blue) is highly enriched around the 3′ end of predicted long 3′ UTRs. Enrichment of a weaker poly(A) signal (red) is also shown, and a randomly chosen sequence (green) is not enriched. (E) Venn diagram indicating tissue-specific expression of 3′-UTR extension events detected in hES cell mRNA.
