Experimental elucidation of the operational genome annotation. (A) Multiple data sets were mapped strand specifically onto experimentally determined structural annotation (gray arrows). Data included peptide reads (data not shown), transcription profiles (green), RNA polymerase binding (red), sigma D binding (blue), and transcription start site reads. Integration of these data sets resulted in genome-wide operon structure (red arrows) with single base pair resolution start site information. (B) Identification of leaderless mRNAs. Position of the TSS matched the start codon (SC). Peptide reads (brown bars) between the TSS and the next possible SC in frame confirmed leaderless mRNA.
