Figure 6.
Strand specificity of eukaryotic DSSS. Coverage plot of reads obtained by paired-end DSSS of a mouse RNA Illumina library with an insert size of 200 nt. The reads were mapped against the reference sequences of the mouse transcriptome, transcript lengths were normalized, and the total number of reads in each of 100 bins per transcript was plotted. Only matches in coding sequences were taken into account. The reads keep the directionality information of the insert. Coverage observed is indicated for PE read 1 (red) and PE read 2 (green). (A) Sense reads; (B) antisense reads.
