Visualization of antisense transcription. Detection of antisense transcription in Mycoplasma using tiling arrays or DSSS. Both technologies detect antisense transcription, but background is much reduced in DSSS. For the sake of clarity, annotated genes are displayed alternately colored purple or yellow. Signal recorded in regions lacking annotation is shown in gray. (A) M. pneumoniae M129 genome, interval 503,000–507,000 bp. Antisense transcription is detected on the forward strand. Reverse strand annotation at this position corresponds to open reading frame mpn420. (B) M129 interval 22,000–24,500 bp. Antisense transcription is detected on the reverse strand. Forward strand annotation at this position corresponds to open reading frames mpn019 and mpn020.
