FISH confirmation. Examples of interphase two-color FISH include three BAC clones. Clones 338M16, 117G16, and 259A1 (red) were identified in CNVR31, 56 and 70, corresponding to WC1.1, ULBP17, and ULBP21, and ATP-binding cassette transporter C4, respectively. Increased signal intensity was confirmed using cohybridization with a unique control BAC clone (297K6, blue) in the same nucleus. These BACs produced variable signal count and/or intensity, 338M16: 3, 2, and 2 and 3; 117G16: 2, 2 and 3, and 2 and 259A1: 2, 3, and 3 in these three cell lines, respectively (for summary, see also Supplemental Table S8). Tandem distribution patterns were most frequently observed. The results of all FISH experiments are available online at http://bfgl.anri.barc.usda.gov/cattleCNV/.
