Table of Contents

ARTICLES

• • High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.

    • F C Lawyer
    • S Stoffel
    • R K Saiki
    • S Y Chang
    • P A Landre

    • First Published May 1, 1993

  • Predominant mutations induced by the Thermococcus litoralis, vent DNA polymerase during DNA amplification in vitro.

    • P Keohavong
    • L Ling
    • C Dias
    • W G Thilly

    • First Published May 1, 1993

  • Rapid characterization of HIV-1 sequence diversity using denaturing gradient gel electrophoresis and direct automated DNA sequencing of PCR products.

    • B Andersson
    • J H Ying
    • D E Lewis
    • R A Gibbs

    • First Published May 1, 1993

  • A PCR-derived library of random point mutations within the V3 region of simian immunodeficiency virus.

    • F Kirchhoff
    • R C Desrosiers

    • First Published May 1, 1993

  • Importance of different variables for enhancing in situ detection of PCR-amplified DNA.

    • G J Nuovo
    • F Gallery
    • R Hom
    • P MacConnell
    • W Bloch

    • First Published May 1, 1993

  • Determination of allele frequencies at loci with length polymorphism by quantitative analysis of DNA amplified from pooled samples.

    • P Pacek
    • A Sajantila
    • A C Syvänen

    • First Published May 1, 1993

  • Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers.

    • G Sarkar
    • R T Turner
    • M E Bolander

    • First Published May 1, 1993

  • Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations.

    • A Takahashi-Fujii
    • Y Ishino
    • A Shimada
    • I Kato

    • First Published May 1, 1993

  • Generation of cohesive ends on PCR products by UDG-mediated excision of dU, and application for cloning into restriction digest-linearized vectors.

    • C Smith
    • P J Day
    • M R Walker

    • First Published May 1, 1993

  • Detection of bovine leukemia virus proviral DNA in individual cells.

    • M L Mirsky
    • Y Da
    • H A Lewin

    • First Published May 1, 1993

  • Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories.

    • G A Penner
    • A Bush
    • R Wise
    • W Kim
    • L Domier
    • K Kasha
    • ...[+1 authors]...,
    • A Laroche
    • G Scoles
    • S J Molnar
    • G Fedak

    • First Published May 1, 1993

  • PCR with degenerate primers containing deoxyinosine fails with Pfu DNA polymerase.

    • T Knittel
    • D Picard

    • First Published May 1, 1993

  • A rapid, semiautomated method for apolipoprotein E genotyping.

    • C Guo
    • P Marynen
    • J J Cassiman

    • First Published May 1, 1993

  • Use of stock solutions to simplify mRNA quantitation by reverse transcription--PCR assays.

    • D Chen
    • V L Magnuson
    • B Steffensen
    • R J Klebe

    • First Published May 1, 1993

  • PCR-based genetic analysis of DNA from autopsied prostate tissue.

    • J A Macoska
    • P D Benson
    • L N Turkeri
    • G P Haas
    • W Sakr

    • First Published May 1, 1993

  • A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.

    • F Poli
    • R Cattaneo
    • L Crespiatico
    • A Nocco
    • G Sirchia

    • First Published May 1, 1993