The association between the levels of candidate instructive factors in RWPE1 and DNA methylation susceptibility, among genes with low transcription in RWPE1. (A) Histone modification levels of genes with different susceptibilities to DNA methylation. For the box plot and statistical methods, refer to the legend to Figure 2B. Active histone modifications were elevated in resistant genes, and H3K27me3 was elevated in susceptible genes. (B) The association between Pol II binding and DNA methylation susceptibility. Pol II binding was associated with resistance even among genes with low transcription. (C) Levels of histone modifications and Pol II binding at various positions against the TSSs in RWPE1. Average levels of histone modifications and Pol II binding of CGIs continuous from their NFRs are shown. (Blue dotted rectangle) The NFRs. (D) The combination effect of one of the three active factors (H3Ac, H3K4me3, and Pol II binding) (y-axis) and H3K27me3 (x-axis) on resistance and susceptibility of genes with low transcription. (Red dots) DNA methylation-resistant genes; (green dots) DNA methylation-susceptible genes; they were separated by any of the three combinations.
