Active HaeIII fragments display features characteristic of transcriptional regulatory elements
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The genomic location relative to the transcription start site (TSS) of the most closely associated gene and vicinity to CpG islands were determined for each of 88 HaeIII DNA sequences isolated from different NFR preparations. The analysis of 23 representative clones is shown. Expression of the associated gene in F9 cells was determined using microarray analysis as described in the text. Distance from the TSS is from the closest end of the HaeIII fragment; NCE indicates noncoding exon; Average length of all 88 HaeIII DNAs is 149 bp.
