(A) H. influenzae global transcription profile. Eighteen H. influenzae tiling path clones were individually transformed in E. coli, the RNA prepared as described in Methods, pooled, and assayed. Clone D was found to be rearranged and was therefore omitted from the pool. Lack of signal in this region further illustrates high probe specificity. Highlighted genes were amplified from the sample by RT-PCR (B). HI0978 was chosen as a negative control since it is located in the tiling path gap between clones K and L. (B) RT-PCR from the H. influenzae RNA pool, targeting the five hyper-transcribed genes highlighted in A. This SYBR Green-stained agarose gel image demonstrates that amplification occurs only when reverse transcriptase is included. EG10978 represents a positive control for E. coli.
