Table 4.
DNA melting curves can be used to calculate the fractional value of domesticus and castaneus alleles of H19 and Kcnq1
Click on table to view larger version.
Domesticus (FVB) and castaneus cDNAs were combined in defined proportions to generate mixes A through K. These cDNAs were amplified and the amplicons subjected to melting analysis using FRET probes as described in Methods. [γDom(Ex)] The fractional value of domesticus cDNA expected based on mixtures generated; (γDomC) experimentally determined fractional values corrected for probe bias; (SD) standard deviations (N = 3); (N/A) not applicable as these samples were used as reference controls.
