Table 1.
DNA melting curves can be used to calculate the fractional value of each allele in a two-allele mixture
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FVB and 129 cDNAs were combined in defined proportions to generate mixes A through K. These cDNAs were amplified and the amplicons subjected to melting analysis as described in Figure 2. [γFVB(Ex)] The fractional value of FVB cDNA expected based on mixtures generated; [γFVB(Ob)] fractional values calculated using Equation 1; (γFVBC) fractional values corrected for biases using Equation 2; (SD) standard deviations (N = 4); (N/A) not applicable because these samples represent the reference standards.
