Callipyge mutation affects gene expression in cis: A potential role for chromatin structure

Imprint analysis of CLPG1 in longissimus dorsi. (A) Nucleotide sequence (using a reverse sequencing primer) of cDNAs generated from two C^MATN^PAT (CN) and two N^MATC^PAT (NC) fetal sheep showing expression of the CLPG1 transcript from both the maternal and paternal alleles, evidenced by the presence of both the T (normal) and C (callipyge) bands at the position of the callipyge mutation (arrowheads). (B) Nucleotide sequence of gDNA and corresponding cDNAs for three C^MATN^PAT and three N^MATC^PAT adult sheep. The gDNA shows the presence of both the T (normal) and C (callipyge) alleles (arrowheads), while the cDNA sequence shows only that the C allele is expressed in adult sheep, regardless of the parental inheritance for that allele.