Expression of CLPG1 in tissues of an adult callipyge sheep. (A) RT-PCR products generated using a gene-specific primer for CLPG1 in the reverse-transcription step. CLPG1-specific amplicons are 270 bp and are designated by the arrowhead. The other bands are nonspecific products of the amplification, confirmed by sequencing. (B) Controls for each reaction in which the reverse transcriptase was omitted. (C) GAPDH amplicons (452 bp) produced using oligo(dT)-primed cDNA from the same RNA samples as those used in A and B. (A,B,C) (lane M) 50-bp marker; (lane 1) longissimus dorsi; (lane 2) biceps femoris; (lane 3) infraspinatus; (lane 4) diaphragm; (lane 5) heart; (lane 6) adrenal gland; (lane 7) kidney; (lane 8) ovary; (lane 9) brain; (lane 10) lung; (lane 11) empty lane; (lane 12) water control. (D) Detection of CLPG1 transcripts in four adult callipyge sheep (NC 2 results represent those shown in panel A). A check-mark indicates detection of the CLPG1 transcript in the designated tissue; a minus sign indicates the transcript was not detected; (nd) not determined.
